Atherosclerosis is a leading cause more mortality and morbidity in worldwide. The underlying pathogenesis involves an imbalanced lipid metabolism and a maladaptive immune response entailing a chronic inflammation of the arterial wall. New pro and anti-inflammatory pathways linking lipid and inflammation biology have been discovered, and genetic profiling studies have unveiled variations involved in human coronary artery disease. The growing understanding of the inflammatory processes and mediators has uncovered an intriguing diversity of targetable mechanisms that can be exploited to complement lipid-lowering therapies. Here we aim to review the recent developments and clinical strategies for the management of atherosclerosis.

High performance thin layer chromatography (HPTLC) offers many advantages over HPLC. It reduces the cost of analysis as compare to HPLC. The mobile phase consumption per sample is extremely low in HPTLC, hence reducing the acquisition and disposal cost. Considering the cost and suitability of analysis for estimation of Domperidone (DMP) in bulk and its marketed formulation, HPTLC method was developed and validated. The Camag HPTLC system, employed with software win CATS (ver.1.4.1.8) was used for the proposed analytical work. Planar chromatographic development was carried out with the help of Silica Gel 60 F₂₅₄ precoated TLC plates. Sample application was facilitated by Linomat 5 applicator. After sample application plates were subjected for ascending development in twin trough chamber of 10×10 dimension, using 10mlof solvent system. The mobile phase was ethyl acetate: methanol (6:4v/v). The detection of spots was carried out densitometrically using a UV detector at 286 nm in absorbance mode. The R_f value for DMP was found to be 0.57-0.64. The HPTLC method was found to be linear across the range 100-700ng/spot. The LOD and LOQ values were 37.72 and 114.30 ng/spot respectively. Performance characteristics of HPTLC method for estimation of DMP in bulk and its marketed dosage form were statistically validated as per the recommendations of ICH guidelines of analytical method validation.

A series of some newly Schiff-base 3a-h and 8 were synthesized by condensation of some aromatic aldehydes with 1, 2, 4-triazole derivatives 2and 6, respectively. The biological screening of the synthesized compounds against microorganism showed that all of them possessing high activity against fungi (Candida albicans). Compounds 3a, 3c, 3f, 3g and 6, show high activity against gram- positive and gram- negative bacteria. Compounds 3d, 3f and 8 showed the highest potency at low µg/ml level against breast carcinoma (MCF-7) and colon carcinoma (HCT116) cell lines respectively. The docking calculations were carried out in order to rationalize the obtained biological results.

Dipteracanthus prostratus nees(Acanthaceae) commonly known as Pottakanchi in Tamil contains a number of phytoconstituents viz, alkaloids phytosterols, glycosides, proteins, amino acids, flavonoids and terpenoids. The objective of the present with solvent study was to isolate and characterize phytoconstituents from the chloroform extract of Dipteracanthus prostratus nees leaves. Chloroform extract was subjected to column. Chromatography and eluted mixtures of increasing polarity composed of benzene and ethylacetate to isolate phytoconstituents. The structure of the isolated compound was established on the basis of elemental analysis and spectroscopic evidences (IR, UV, ¹HNMR,^13CNMR, MS) A β-sitosterol was isolated from the chloroform extract of the leaves of the plant. The yield of compound was 0.056% w/w m.p 136⁰C to 138⁰C. λ max in EtOH: 212nm, Rf value 0.4 in {toluene: ether: cyclohexane} (5:2:1) Dipteracanthus prostratus nees contains β-sitosterol which may be responsible for various pharmacological activities of the plant.

The present research work discusses the development of UV Spectroscopic method for the estimation of Linezolid. Simple, specific, accurate and cost effective spectroscopic method has been developed for the estimation of Linezolid in bulk as well as formulation. The optimum conditions for the analysis of the drug were established. The maximum wavelength (λ_max) was found to be 251nm. The validation was performed as per ICH guidelines for linearity, accuracy, precision, LOD and LOQ. The method shows high sensitivity with linearity in the range of 1-6 µg/ml and shows a linear relationship between the absorbance and concentration with coefficient of correlation 0.999. The regression of curve was Y = 0.0563x + 0.0211. The precision of method was found to be good. The percentage recovery was found to be 99.47 ± 0.33. The optimized showed good reproducibility and recovery with RSD < 2%. The proposed method will be suitable for analysis of Linezolid in bulk as well as pharmaceutical formulations in quality control purpose. It is thus concluded that the proposed method is new, simple, cost effective, safe, accurate, precise and environmental friendly.