A simple, rapid, precise and highly selective spectrophotometric method was developed for simultaneous estimation of Domperidone and Pantoprazole sodium in pure as well as tablet dosage form. The simultaneous equation method is based on measurement of absorbance at 288 nm and 291 nm as two wavelengths selected for quantification of Domperidone and Pantoprazole sodium using methanol as a solvent. The method was validated for specificity, linearity, accuracy, precision, robustness and ruggedness. A double-beam shimadzu UV-visible spectrophotometer, 1800 with a pair of 1 cm matched quartz cells was used to measure the absorbance of the solutions in developed method. The method was validated as per ICH guidelines. Linearity ranges from 5-25 µg/ml for Domperidone and 5-25 µg/ml for Pantoprazole of the drugs. % RSD calculated was less than equal to 2 which indicates accuracy and reproducibility of the method. Recovery study indicates that these drugs could be quantified simultaneously without interference of excipient present in formulation. The developed UV spectroscopic method is suitable for the analysis of DMP and PTZ in combined dosage form. The accuracy was found between 99-100% for DMP and 98-99% for PTZ respectively. The precision (% RSD) was found to be 0.308 for DMP and 0.123 for PTZ respectively. The LOD was found to be 0.045µg/ml for DMP and 0.01µg/ml for PTZ respectively. The LOQ was found to be 0.122µg/ml for DMP and 0.059µg/ml for PTZ respectively.

A simple, rapid, precise and highly selective spectrophotometric method was developed for simultaneous estimation of Tartrazine and Sunset yellow in pure as well as foodstuffs. The simultaneous equation method is based on measurement of absorbance at 427nm and 483 nm as two wavelengths selected for quantification of Tartrazine and Sunset yellow using distilled water as a solvent. The method was validated for specificity, linearity, accuracy, precision, robustness and ruggedness. A double-beam shimadzu UV-visible spectrophotometer, 1800 with a pair of 1 cm matched quartz cells was used to measure the absorbance of the solutions in developed method. The method was validated as per ICH guidelines. Linearity ranges from 5-25µg/ml for Tartrazine and 5-25 µg/ml for Sunset yellow of the dyes. % RSD calculated was less than equal to 2 which indicates accuracy and reproducibility of the method. Recovery study indicates that these drugs could be quantified simultaneously without interference of excipient present in formulation. The developed UV spectroscopic method is suitable for the analysis of TAR and SY in combined foodstuffs.

A novel validated Reverse phase HPLC method for the simultaneous determination of Paracetamol and Flupirtine maleate in pharmaceutical dosage form was developed and validated. Proposed research work was developed by selecting Inertsil, C18, (250 x 4.6mm, 5µ) column as stationary phase and Methanol: Ortho Phosphoric acid (65:35 v/v) as mobile phase. Separation was achieved at flow rate of 1mL/ min at ambient temperature throughout the experiment. Quantification was achieved with ultraviolet (PDA) detection at 280 nm. The retention times of Paracetamol and Flupirtine maleate were found as 4.7 min and 3.37 min respectively. The linearity concentration of 50-150μg/mL for both Paracetamol and Flupirtine maleate respectively, and the regression coefficients found as 0.998 and 0.999 for Paracetamol and Flupirtine maleate respectively. The % recovery of pharmaceutical formulations for both within the accepted limits and there is no interference with any excepients in the formulation. This method was validated according to ICH guidelines.

A RP-HPLC method has been developed for the estimation of Salmeterol Xinafoate in bulk and formulation. The Chromatographic separation has performed with Phenomenex, C8 column (150 x 4.6 mm, 5µm) and mobile phase, Acetonitrile: Water (10:90v/v). The flow rate was 1ml/min and eluent were monitored at 216 nm. The retention time of Salmeterol Xinafoate was 3.6 min. The method was found to be linear over a range of 5-25 µg/ml for Salmeterol Xinafoate with correlation coefficient (r2= 0.9991). The validation results showed that the method is reproducible, precise and has satisfactory accuracy and linearity profile for the assay of Salmeterol Xinafoate. So, the methods can be successfully applied for the routine analysis of Salmeterol Xinafoate. The degradation studies indicated that Salmeterol Xinafoate showed degradation in acid.

A rapid, simple, selective, sensitive, precise and specific UV Spectrophotometric method has been developed for the determination of Fluocinolone acetonide in bulk and(200-400nm) in 1cm quartz cell in a double beam UV Spectrophotometer. The spectrophotometric detection was carried out at an absorption maximum of 235 nm using Methanol as solvent. The detector response for the Fluocinolone acetonide was linear over the selected concentration range 1.25-6.25µg /ml with a correlation coefficient of 0.999and equation for the regression curve was found to be y=0.1596x + 0.0175. The accuracy was between 99-100%. The precision (%RSD) among six samples preparation was 0.121%.The LOD and LOQ was 0.1125 and 0.309µg /ml respectively. Statistical analysis proved that the methods are repeatable and specific for the determination of the said drug. These methods can be adopted in the routine assay analysis of Fluocinolone acetonidein bulk and pharmaceutical dosage form.